Evaluation Of Propolis Extract As A Natural Antimicrobial Agent Against Escherichia Coli
DOI:
https://doi.org/10.64354/ofon.ci.2.1.51Abstract
Propolis extract's antibacterial efficacy against bacterial isolates taken from enteritis patients was assessed in this study, with an emphasis on multidrug-resistant (MDR) Escherichia coli. The Vitek-2 compact system, morphological, biochemical, and culture techniques were used to identify Gram-positive and Gram-negative bacteria from a total of 30 clinical samples. E. Coli accounted for 30% of all Gram-negative pathogens, with Klebssiella pneumonia and Proteus mirabilis following closely after. High rates of resistance to various widely used antibiotics, such as erythromycin, doxycycline, colistin, and piperacillin, were found in E. coli isolates by antibiotic susceptibility testing; nevertheless, ciprofloxacin and cefotaxime continued to exhibit notable action. Using the disc diffusion method, propolis extract was produced in varying quantities and tested against isolates of MDR E. coli. The findings showed that propolis had a definite dose-dependent inhibitory effect, with certain isolates exhibiting inhibition zones that were on par with or greater than those of ciprofloxacin. At 100 mg/ml, the greatest action was seen, resulting in inhibition diameters of up to 26 mm. These results suggest that propolis has promising antibacterial properties and could be used as a supplemental or natural medicinal treatment to combat enteritis-causing bacteria that are resistant to antibiotics.
References
Reference
1-Ahangari, Z.; Naseri, M.; Vatandoost, F. Propolis (2018): Chemical Composition and Its Applications in Endodontics. Iran. Endod. J. , 13, 285–292.
2-Przybyłek, I.; Karpiński, T.M .( 2019). Antibacterial Properties of Propolis. Molecules , 24, 2047.
3-Bankova, V.; Popova, M.; Trusheva, B .( ( 2006, Plant Sources of Propolis: An Update from a Chemist’s Point of View. Nat. Prod. Commun. 1, 1023–1028.
4-Souza, Ε.A.; Zaluski, R.; Veiga, N.; Orsi, R.O. (2016), Effects of Seasonal Variations and Collection Methods on the Mineral Composition of Propolis from Apis mellifera linnaeus Beehives. Braz. J. Biol. 76, 396–401.
5- Pokharel, P., Dhakal, S., & Dozois, C. M. (2023). The diversity of Escherichia coli pathotypes and vaccination strategies against this versatile bacterial pathogen. Microorganisms, 11(2), 344.
6- Geurtsen, J., de Been, M., Weerdenburg, E., Zomer, A., McNally, A., & Poolman, J. (2022). Genomics and pathotypes of the many faces of Escherichia coli. FEMS microbiology reviews, 46(6), fuac031.
7- Migliori, G. B., Marx, F. M., Ambrosino, N., Zampogna, E., Schaaf, H. S., Van Der Zalm, M. M., ... & Visca, D. (2021). Clinical
standards for the assessment, management and rehabilitation of post-TB lung disease. The International Journal of Tuberculosis and Lung Disease, 25(10), 797-813.
8- Hendriks, F., Rojas-Lopez, R. R., Koopmans, B., & Guimarães, M. H. (2024). Electric control of optically-induced magnetization dynamics in a van der Waals ferromagnetic semiconductor. Nature Communications, 15(1), 1298.
9- Martinez-Medina, M., Strozzi, F., Ruiz Del Castillo, B., Serrano-Morillas, N., Ferrer Bustins, N., & Martínez-Martínez, L. (2020). Antimicrobial resistance profiles of adherent invasive escherichia coli show increased resistance to βlactams. Antibiotics, 9(5), 2
10- Tillier, A., & Abdelhalim Ali, A. (2021). Qous (2020). Bulletin archéologique des Écoles françaises à l’étranger.
11- Lucchini, V. (2020). Tackling bacterial pathogenesis: a transcriptional regulator as novel target for antivirulence therapy (Doctoral dissertation, University_of_Basel)
12- Mirsepasi-Lauridsen, H. C., Vallance, B. A., Krogfelt, K. A., & Petersen, A. M. (2019). Escherichia coli pathobionts associated with inflammatory bowel disease. Clinical microbiology reviews, 32(2), 10-1128.
13- Clermont, O., Condamine, B., Dion, S., Gordon, D. M., & Denamur, E. (2021). The E phylogroup of Escherichia coli is highly diverse and mimics the whole E. coli species population structure. Environmental Microbiology, 23(11), 7139-7151.
14- MacFaddin,. J.F. (2000). Biochemical test for identification of medical bacteria. 2 ed. William and Wilkins. Comp. Baltimore, p.490.
15- Collee, J. G. fraser, AG; Marmion, BP and Simmons, A.(1996). Mackie and McCartney, practical Medical Microbiology, 14th edn. Churchil living stone, New York
16- Forbes ,B.A. , et. al. (2007). Bailey and Scott's Diagnostic microbiology . 12th ed Mosby Elsevier company , USA Carlone, G. M.; Thomas , M.; Rumschlag, H.; and Sottnek, F. Chapter Five References 98 (1986). Rapid micro procedure for isolation detergent insoluble OMP. from Hib. Clin. Microb. J. 24(3): 330-332
17- Freeman, D. J., Falkiner, F. R., & Keane, C. T. (1989). New method for detecting slime production by coagulase negative staphylococci. Journal of clinical pathology, 42(8), 872-874
18- Dalhoff, A. (2012). Global fluoroquinolone resistance epidemiology and implictions for clinical use. Interdisciplinary perspectives on infectious diseases, 2012
19- Kanti, p. Evaluation of antimicrobial screenin extract of some selected dye yiel.
20- Arbabi, E., Arbabi, A., Kamali, S. M., Horie, Y., & Faraon, A. (2016). Multiwavelength metasurfaces through spatial multiplexing. Scientific reports, 6(1), 32803
21-Remy, J. M.; Tow-Keogh, C. A.; McConnell, T. S.; Dalton, J. M., and Devito, J. A. (2012). Activity of delafloxacin against methicillin-resistant Staphylococcus aureus: resistance selection and characterization. Journal of antimicrobial chemotherapy, 67(12), 2814-2820
22-- Luthje, P. and Brauner, A. (2014). Virulence factors of uropathogenic E. coli and their interaction with the host. Adv. Microb. Physio. 1 65, 337-372.
23- Hossain, A., Hossain, S. A., Fatema, A. N., Wahab, A., Alam, M. M., Islam, M. N., ... & Ahsan, G. U. (2020). Age and gender-specific antibiotic resistance patterns among Bangladeshi patients with urinary tract infection caused by Escherichia coli. Heliyon, 6(6), e04161
24- Maraki, S.; Mantadakis, E.; Michailidis, L. and Samonis, G. (2013). Changing antibiotic susceptibilities of community-acquired uropathogens in Greece, 2005–2010. Journal of Microbiology, Immunology and Infection, 46(3), 202-209.
25- Sultan, M., Muhammad, A., Ullah, M., Sadiq, N., Akhtar, K., & Akbar, H. (2012). cardiac catheterization in grown-up patients with congenital heart diseases–indications and complications. Pakistan Heart Journal, 45(3).
26-Nalini, R.; Ramya, J. E.; Meenakshi, B.; Palniappan, N. and Poongodi, S. (2014). Recent Sensitivity Pattern of Escherichia coli in Urinary Tract Infection. RRJMB. 3: 3.
27-Sumera Sabir, Aftab Ahmad Anjum, Tayyaba Ijaz, Muhammad Asad Ali, Muti ur Rehman Khan, Muhammad Nawaz. (2014). Isolation and antibiotic susceptibility of E. coli from urinary tract infections in a tertiary care hospital, Pak J Med Sci Vol. 30 No. 2,389-392
28-Kibret ,M. and Abera, B. (2011). Antimicrobial Susceptibility Patterns of E. coli from Clinical Sources in Northeast Ethiopia. Afr Health Sci. 11(1): 40 – 45. Cureus. 9(6): 1-9.
29-M’Koma, A. E. (2018). The multifactorial etiopathogeneses interplay of inflammatory bowel disease: an overview. Gastrointestinal Disorders, 1(1), 75- 105.
30-Mohammed, B. I., & Amin, B. K. (2022). Sociodemographic characteristics, smoking, and family history of patients with inflammatory bowel disease, northern part of Iraq. Medical Journal of Babylon, 19(4), 615-619.
31-Walton, H., Gros, B., & Din, S. (2024). Crohn's disease. Medicine.-
32-Parvez, S. A., & Rahman, D. (2018). Virulence factors of uropathogenic E. coli. Microbiology of Urinary Tract Infections-Microbial Agents and Predisposing Factors, 7-21
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Chemical Interactions
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
