Spectrophotometric Analysis for Sulfamethoxazole Estimation Employing Potassium Permanganate as an Oxidant: A Study Exploring the Impact of Experimental Variables Using Design of Experiments and ANOVA
DOI:
https://doi.org/10.64354/mkghn626Keywords:
Sulfamethoxazole, Kinetic spectrophotometry , Oxidation, Potassium permanganate , ANOVAAbstract
A selective and rapid spectrophotometric method for the determination of sulfamethoxazole was developed based on its oxidation with potassium permanganate in acidic medium. The effect of various crucial experimental parameters such as oxidant concentration, acid concentration, reaction temperature, and reaction time were examined. A 2³ full factorial design was employed to evaluate the individual effects of these factors as well as their interactions on the absorbance of the oxidation product. The maximum absorbance, the optimum temperature, and the optimal reaction time were determined, and ANOVA-based statistical analysis was performed to assess the significance of the studied variables. The developed procedure can be effectively applied for the quantitative determination of sulfamethoxazole in bulk drug, pharmaceutical formulations, and environmental water samples. The proposed method is simple, sensitive, precise, economical, and time-saving, and is suitable for routine analysis of sulfamethoxazole in pharmaceutical and environmental samples.
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